|Presentation of the Microarrays Tissue|
“Tissue MicroArray” (TMA) is a technique that allows bringing together, on one single paraffin block, samples from different tissue sections already embedded into other paraffin blocks.
For instance, one paraffin block can bring together:
- samples of the same tumor type from distinct patients,
- samples from a single patient but taken in different locations,
- samples of different tissues, normal or pathological, from a single patient.
This technique allows analyzing simultaneously the expression of various proteins coming most of the time from several hundreds of tumoral samples placed on a single block. The paraffin-embedded block qualified as "receiver", contains biopsic cylinders (usually with a diameter about 600 microns) stemming from paraffin-embedded blocks of hundreds of different tumoral samples considered as "donors" samples.
Thus TMA allow:
- reducing the technical time required for the cutting and immunohistochemical analysis of the samples,
- reducing the quantity of consumables (for instance antibodies) necessary to prepare the whole samples,
- standardize the technics (absence of inter-technician or intra-technician variations for instance),
- a semi-automated processing of the data, using the appropriate equipment and software.
TMA Request Procedure
1/ Go the TMA section and search for TMA for your research projects, based on criteria such as organ, pathology, etc
2/ Indicate the ID number of the TMA of interest (as well as the pathology their referring to) in the TMA Request Form
3/ Make your request by sending an email to firstname.lastname@example.org providing the list of selected samples that you've exported, along with the TMA Request Form filled out.
The 3-D pie charts below show the distribution of TMA per institution and per pathology.